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Sequence localizer/oligonucleotide finder

This application can be used to search for a sequence or motif within a DNA sequence. It is great for localizing oligos within a target sequence, to "manually" look for restriction sites, and to locate binding sites for transcription factors or other DNA-binding proteins with a know binding consensus sequence.

The application fully supports degenerated sequences, both in the query and in the target sequence. Degeneration is managed through IUPAC code, that allows to specify ambiguous characters.

If several matches are found, details are provided for each match, with start and end points and the actual sequence matched.

Input target sequence is limited to 100.000 base pairs to limit server load

TARGET SEQUENCE

Paste your DNA sequence below in any format

QUERY SEQUENCE

Paste your search pattern/oligo here
You can use a 'degenerated' or ambiguous code to match different sequences that match the same pattern (see IUPAC code).
Example: AANNGAATTGK, will match any nucleotide (or ambiguous IUPAC nucleotide!!) in positions 3-4 and G or T in the last position


A web application written in Python by Andrea Cabibbo